Food Fraud is a growing problem for food industry that is costing crores of rupees. One of the greatest problems facing this branch of criminal investigation - FOOD FORENSICS - is that consumers can't always tell when they are being defrauded. The product might taste similar, will probably look similar, will cost about the same, will not necessarily do the consumer any harm.

A variety of techniques including DNA-based methods are used to identify grains in food mixtures, and to fingerprint grain varieties.


Basic principle or theory is that, all living cells have a particular DNA in their mitochondria. This DNA - cytochrome oxidase DNA is specific to a particular speices. Moreover, this DNA is not damgaed during the extreme processing conditions so it can be retained and even detected in processed food.


I would like to have a thought on this and imagine how this techniique can be used.

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Comment by Nitin Rana on May 17, 2011 at 5:31am
Comment by Nitin Rana on May 17, 2011 at 4:17am

Thats gr8..nw fp is speaking the mega structure DNA......

well u r correct with the advancement in the molecular diagnostic kits we can identify the contaminants with in few mins....besides this the DNA fingerprinting is used to detect as well as quantify the small grains added.......technically its named DNA identikit....Use of DNA barcode & fingerprinting is common...

The use of DNA barcodes is widespread in clinical microbiology and I am describing here only one of many applications. Very rapid detection of viral pathogens was achieved using a bio-barcode with a capillary DNA analyser, which allowed identification of the viral pathogen at very low levels within a few minutes. An automated system described as a universal pathogen biosensor capable of identifying bacteria, viruses, fungi and protozoa was developed with funding from the United States Department of Defence. The technology uses mass spectrometer derived base-composition signatures obtained from PCR amplification of pathogen genomes to identify most organisms present in an environmental or tissue sample. The system uses computer software to handle the mass of information.  The instrumental package called the Ibis Universal Biosensor is an automated platform for pathogen identification and strain typing.

 

DNA fingerprinting traced the origin of a patented yellow bean variety Enola to an older Mexican variety, and Enola clearly lacked differences to distinguish it from the traditional Mexican cultivar. DNA-based methods were used to identify grains in food mixtures, and to fingerprint grain varieties. DNA fingerprinting identified the fruit tree species used in selection and introgression of new varieties. DNA fingerprinting of microbial plant pathogens proved useful in diagnosis and disease management. DNA fingerprinting of human pathogens such as the tuberculosis bacterium for over a decade has proven a powerful epidemiological tool. The technique is valuable for establishing the lineages of animal breeding stock and perhaps even more so in identifying cloned animals and their progeny  as they are offered for sale or export.

http://www.i-sis.org.uk/DNAidentikit.php

I have selected a nice paper

http://engrwww.usask.ca/oldsite/societies/csae/protectedpapers/c062...

Comment by Pankaj Sharma on May 15, 2011 at 6:42pm

Also it would be interesting to see that how seriously govt. thinks over constituting a seperate body for it as we all know that current deptts, especially the Food Inspection deptts. are much corrupted and don't act seriously on food and health related issues, reasons being many. A seperate body will not only ensure the fair quality assurance of food products but will also help in building consumer's trust over this as most of them simply treat govt. related work as another profit minded activity.

With Regards,
Pankaj 

Comment by Anusha Ramani on May 14, 2011 at 11:54pm

The method, is indeed useful, but i think will be very costly. A comparative analysis with  rest of the methods prevailing in food science can surely proove its requirement and its advantage to others. Cartainly, it has firstly opened more avenues for research purpose, before it can actually be implemented commercially.

Comment by Prashant Sahni on May 13, 2011 at 4:04pm
sir this sounds something novel , will be looking forward for some more information on this topic , thanks

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